CFX96TOUCH Fluorescent Quantitative PCR akụrụngwa

Enwere ike iji PCR CFX96Touch fluorescent quantitative na mpaghara nyocha dị iche iche nke ọnụọgụ nucleic acid, nyocha ọkwa mkpụrụ ndụ ihe nketa, nchọpụta mkpụrụ ndụ ihe nketa, nchọpụta GMO, na nyocha kpọmkwem ngwaahịa.

gburugburu ọrụ:
1.1 Ọnọdụ okpomọkụ: 5-31 Celsius C
1.2 Na-arụ ọrụ na iru mmiri: iru iru mmiri ≤80%
1.3 Ike ịrụ ọrụ: 100-240 VAC, 50-60Hz
Arụmọrụ na teknụzụ chọrọ nke CFX96Touch fluorescence quantitative PCR
3.1 Isi arụmọrụ (* bụ egosi nke a ga-ezute)
* 3.1.1 Ọwa ule isii, 5% PCR nwere ike mezuo, yana mkpụrụ ndụ ihe nketa 5 nwere ike ịchọpụta n'otu oge, yana ọwa nchọpụta FRET pụrụ iche na-achọpụta n'otu oge.
* 3.1.2 Site na ọrụ PCR gradient okpomọkụ dị ike, ị nwere ike ịgba ọsọ 8 dị iche iche n'otu oge, incubation okpomọkụ ọ bụla.
3.1.3 mmeghe nke reagents zuru oke, nyocha dị iche iche na reagents ụlọ ọgwụ na-etinye;
3.1.4 Kwesịrị ekwesị maka ụzọ dị iche iche nke fluorescence dị ka Taqman, Molecular Beacon, Fret probe, Sybr Green i, wdg .;
3.1.5 Mepee, 0.2ml otu tube, octal, efere 96-ọma, wdg.
* 3.1.6 nwere ike na-agba ọsọ n'onwe ya, n'ezie offline ọrụ, ọ dịghị mkpa jikọọ na kọmputa ka nyochaa PCR fluorescent amplification curve ozugbo;
3.2 Isi teknụzụ chọrọ (* bụ ihe ngosi a ga-emezurịrị)
* 3.2.1 Sample ikike: 96 × 0.2ml, ọkọlọtọ nkọwa 96-ọma efere (12×8) nwere ike iji;
3.2.2 Supply Type: 0.2ml otu tube, asatọ interlocking, 96-ọma efere, wdg.
3.2.3 Usoro mmeghachi omume: 1-50μL (10-25 μL kwadoro);
* 3.2.4 Isi iyi ọkụ: LED isii nwere nzacha;
* 3.2.5 Onye nchọta: diode foto isii nwere nzacha;
* 3.2.6 lita jụrụ oyi: 5 Celsius C / sec;
3.2.7 Oke njikwa okpomọkụ: 0 -100 Celsius;
3.2.8 Okpomọkụ ziri ezi: ± 0.2 ° C (90 ˚C);
3.2.9 Ụdị ọnọdụ okpomọkụ: ± 0.4 ° C (90 ˚C n'ime 10 sekọnd);
* 3.2.10 Ọrụ gradient na-agbanwe agbanwe: Gbaa okpomọkụ 8 dị iche iche n'otu oge; oke njikwa okpomọkụ gradient: 30 -100 Celsius; ogo dị iche iche okpomọkụ gradient: 1 - 24 ° C; gradient okpomọkụ incubation oge: otu;
3.2.11 mkpali mkpali / ikuku ikuku ogologo: 450-730 nm;
3.2.12 Mmetụta: Enwere ike ịhụ otu mkpụrụ ndụ mkpụrụ ndụ ihe nketa na genome mmadụ;
3.2.13 Oke dị ike: 10 ọnụọgụ;
3.2.14 Ngosipụta: 8.5 inch agba ihuenyo mmetụ;
3.2.15 Data Analysis Mode: Standard Curve Quantity, Melting Curve, CT ma ọ bụ ΔΔCT Gene Expression Analysis, Multiple Internal Geodes Analysis and Amplification Exfaciency Calculation, Multiple Data Files Gene Expression Analysis, Allelic Analysis, Ụdị Analysis, Nwere Gene, na-agbaze ọrụ nyocha nyocha. ;
3.2.16 Mbupụ data: Excel, Okwu, ma ọ bụ PowerPoint. Akụkọ onye ọrụ nwere ntọala ọsọ, eserese na nsonaazụ data tebụl, nke enwere ike ibipụta ma ọ bụ chekwaa dị ka PDF;
* 3.2.17 Ihe omumu ihe omumu nke chromosomal: Usoro nyocha nke onu ogugu nke ihe owuwu chromatin site na ntule nke mmebi DNA genomic site na ntule nke DNA genomic. Ọ na-egosipụta n'ezie njikọ dị elu n'etiti usoro chromatin na okwu mkpụrụ ndụ ihe nketa;