Dị ka ụkpụrụ nke Kjeldahl nitrogen kpebisiri ike, a chọrọ nzọụkwụ atọ maka mkpebi siri ike, ya bụ mgbaze, distillation na titration.
Mgbari: Kpoo ihe ndị nwere nitrogen nwere ogige (protein) yana sulfuric acid gbadoro anya na ihe na-eme ka ọ dị ike (ọla kọpa sulfate ma ọ bụ mbadamba mgbaze Kjeldahl) iji mebie protein ahụ. A na-etinye carbon na hydrogen ka ọ bụrụ carbon dioxide na mmiri iji gbanarị, ebe a na-agbanwe Nitrogen organic ka ọ bụrụ amonia (NH3) ma jikọta ya na sulfuric acid ka ọ ghọọ ammonium sulfate. (Ammonium NH4+)
Usoro mgbaze: kpo oku na obere okpomọkụ ka obụpde, ihe dị na flask bụ carbonized na oji, na-emepụta nnukwu ụfụfụ. Mgbe ụfụfụ ahụ ga-apụ n'anya, mụbaa ike ọkụ iji nọgide na-enwe ntakịrị esi mmiri. Mgbe mmiri mmiri na-acha anụnụ anụnụ-acha akwụkwọ ndụ ma doo anya, nọgide na-ekpo ọkụ maka 05-1h, ma dị jụụ mgbe njedebe gasịrị. (Ị nwere ike iji ngwa mgbaze na-akpaghị aka iji rụchaa ọrụ nhazi tupu)
Distillation: A na-agbaze ihe ngwọta enwetara na olu mgbe niile wee tinye ya na NaOH iji hapụ NH3 site na distillation. Mgbe condensation gasịrị, a na-anakọta ya na ngwọta boric acid.
Usoro nhazi: Nke mbụ, a na-agbaze ihe nlele ahụ gbazere, a na-agbakwunye NaOH, na gas amonia na-emepụta mgbe ikpo ọkụ na-abanye na condenser, na-asọba n'ime karama nnabata nke nwere ngwọta boric acid mgbe emechara ya. Ụdị ammonium borate. (A na-agbakwunye ihe ngosi agwakọtara na ngwọta boric acid. Mgbe emechara ammonium borate, ihe ngwọta na-amịkọrọ na-agbanwe site na acidic gaa na alkaline, na agba na-agbanwe site na odo odo gaa na-acha anụnụ anụnụ.)
Titration: Titrate na ihe ngwọta ọkọlọtọ hydrochloric acid nke ntinye amaara ama, gbakọọ ọdịnaya nitrogen dabere na ọnụọgụ hydrochloric acid riri, wee mụbaa ya site na ihe ngbanwe kwekọrọ iji nweta ọdịnaya protein. (Titration na-ezo aka na usoro nke quantitative analysis na kwa a chemical nnwale ọrụ. Ọ na-eji quantitative mmeghachi omume nke abụọ ngwọta iji chọpụta ọdịnaya nke a ụfọdụ solute. Ọ na-egosi njedebe njedebe nke titration dị ka agba mgbanwe nke egosi, wee jiri anya hụ oriri ọkọlọtọ ọkọlọtọ Mpịakọta, mgbako na nsonaazụ nyocha.)
Usoro titration: Wepụ ọkọlọtọ ọkọlọtọ nke hydrochloric acid n'ime ngwọta ammonium borate iji gbanwee agba nke ngwọta site na-acha anụnụ anụnụ-acha akwụkwọ ndụ na-acha uhie uhie.
DRK-K616 akpaka Kjeldahl nitrogen analyzer bụ onye nyocha nwere ọgụgụ isi na-akpaghị aka maka mkpebi ọdịnaya nitrogen dabere na usoro Kjeldahl. Enwere ike iji ya mee ihe n'ọtụtụ ebe na nhazi nri, mmepụta nri, ụtaba, ịzụ anụmanụ, fatịlaịza ala, nlekota gburugburu ebe obibi, ọgwụ, ọrụ ugbo, nchọpụta sayensị, nkuzi, nlekọta dị mma na mpaghara ndị ọzọ maka nyocha nke nitrogen na protein na nnukwu na ọkara micro. ihe atụ. A pụkwara iji ya mee ihe maka nnu ammonium, Nchọpụta nke acid fatty / alkali na-agbanwe agbanwe, wdg Mgbe ị na-eji usoro Kjeldahl chọpụta ihe nlele ahụ, ọ kwesịrị ịgafe usoro atọ nke mgbaze, distillation, na titration. Distillation na titration bụ isi usoro nha nke DRK-K616 Kjeldahl nitrogen analyzer. Ụdị DRK-K616 Kjeldahl nitrogen analyzer bụ usoro ntugharị ikuku na-akpaghị aka na titration nitrogen nke emebere dị ka usoro mkpebi Kjeldahl nitrogen si dị; ngwá ọrụ a na-enye ohere dị ukwuu maka ndị nyocha ụlọ nyocha na usoro nke ịchọpụta nitrogen-protein. , Na nwere njirimara nke nchekwa na ntụkwasị obi ojiji; ọrụ dị mfe na-azọpụta oge. Igwe okwu mkparịta ụka ndị China na-eme ka onye ọrụ dị mfe ịrụ ọrụ, interface ahụ dị enyi, na ozi egosipụtara bara ụba, ka onye ọrụ wee nwee ike ịghọta ngwa ngwa iji ngwá ọrụ ahụ.
Oge nzipu: Feb-25-2022
